This paper examines the effectiveness of two pretreatments on Source-Separated Organic waste (SSO) mixed with wood wastes: long term lime for SSO mixed with forestry waste (hardwoods), and the cellulose solvent-organic lignocellulose fractionation (COSLIF) method, with SSO and demolition waste (softwoods). For long term lime treatment, the highest overall conversions from cellulose to glucose and xylose were 50.4%, and 43.5% respectively. The best temperature found for long term lime pretreatment was 65°C. The COSLIF pretreatment glucose yield was found to be 93.7%. The highest enzyme hydrolysis yield found was 93.5% for a cellulase loading of 30 FPU/g glucan at 50°C. The best hydrolysis yield found at lower loading (10 FPT/g glucan), was 83.5%. At 40 and 50°C, all peak hydrolysis yields were achieved between 12 and 24 hours. A drop in temperature below 40°C caused a slowing of the hydrolysis rate.