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Feasibility of Establishing an Enzyme-Linked Immunosorbent Assay (ELISA) Laboratory for the Detection of Dioxins in Economically Marginalized and Developed Countries

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posted on 2023-06-19, 15:56 authored by Adrienne K. Lee
Finding a low cost and accessible means of detecting for dioxins in contaminated soil is a necessary step to ensure the health and safety of humans and the environment worldwide. Conventional technologies based on mass spectrometry are expensive and inaccessible. A minimally resourced laboratory and the use of ELISA will be discussed as a feasible, accessible, low cost alternative. The correlation between a minimally resourced laboratory (Ryerson University) and a fully resourced laboratory (Ontario Ministry of the Environment) was strong (n=13, r²=0.888, slope=0.87). To demonstrate the functionality of the minimally resourced laboratory, a supplemental site was characterized using ELISA. Results from the Ryerson and OMOE laboratories produced similar dioxin concentrations of undetectable to 120.26pgTEQg⁻¹ and 32.38 to 163.2pgTEQg⁻¹, respectively. This study illustrates an alternative for evaluating contaminated soil that could serve as a technology transfer for marginalized economies, and provide an accessible form of sample analysis in developed countries.

History

Language

eng

Degree

  • Master of Applied Science

Program

  • Environmental Applied Science and Management

Granting Institution

Ryerson University

LAC Thesis Type

  • Thesis

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    Environmental Applied Science and Management (Theses)

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